Denaturing Gradient Gel Electrophoresis as a Tool To Determine Batch Similarity of Probiotic Cultures of Porcine Cecal Bacteria

Michael Hume; Charles M. Scanlan; Roger B. Harvey; Kathleen Andrews; James D. Snodgrass; Armen Nalian; Alexandra Martynova-Van Kley; David Nisbet

doi:10.1128/aem.02580-07

Denaturing Gradient Gel Electrophoresis as a Tool To Determine Batch Similarity of Probiotic Cultures of Porcine Cecal Bacteria

Michael Hume, Charles M. Scanlan, Roger B. Harvey, Kathleen Andrews, James D. Snodgrass, Armen Nalian, Alexandra Martynova-Van Kley, David Nisbet

Stephen F Austin State University

Research output: Contribution to journal › Article

Abstract

A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

Original language	Undefined/Unknown
Journal	Faculty Publications
DOIs	https://doi.org/10.1128/aem.02580-07
State	Published - Jan 1 2008

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Denaturing Gradient Gel Electrophoresis as a Tool To Determine Ba

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Cite this

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title = "Denaturing Gradient Gel Electrophoresis as a Tool To Determine Batch Similarity of Probiotic Cultures of Porcine Cecal Bacteria",

abstract = "A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.",

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AU - Andrews, Kathleen

AU - Snodgrass, James D.

AU - Nalian, Armen

AU - Kley, Alexandra Martynova-Van

AU - Nisbet, David

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AB - A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

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DO - 10.1128/aem.02580-07

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